https://divingintogeneticsandgenomics.com/tags/tophat/ Recent content in Tophat on Chatomics Hugo -- gohugo.io en-us © 2018 Ming 'Tommy' Tang Tue, 17 Sep 2013 11:49:00 -0700 https://divingintogeneticsandgenomics.com/archive/tophat-run-time/ Tue, 17 Sep 2013 11:49:00 -0700 https://divingintogeneticsandgenomics.com/archive/tophat-run-time/ It took me 45 hrs to map a 24G single-end RNA-seq data to human hg19 with the gtf file from GENECODE! I run tophat on a cluster with 1 node, 8 processors with 3gb ram for each processor (the max free resource as a regular user). I have six such files...it will take me 12 days to finish the mapping....wish I had better access to the computing resource... a quick google confirmed that it does take that long.