https://divingintogeneticsandgenomics.com/tags/sambamba/ Recent content in Sambamba on Chatomics Hugo -- gohugo.io en-us © 2018 Ming 'Tommy' Tang Thu, 05 May 2016 10:41:00 -0700 https://divingintogeneticsandgenomics.com/archive/downsampling-bam-files-to-a-certain-number-of-reads/ Thu, 05 May 2016 10:41:00 -0700 https://divingintogeneticsandgenomics.com/archive/downsampling-bam-files-to-a-certain-number-of-reads/ When compare different ChIP-seq data sets or analyze a set of ChIP-seq data sets together (e.g. ChromHMM analysis), it is desirable to subsample the deeply sequenced ones to a certain number of reads (say 15million or 30 million). In paper Integrative analysis of 111 reference human epigenomes: To avoid artificial differences in signal strength due to differences in sequencing depth, all consolidated histone mark data sets (except the additional histone marks the seven deeply profiled epigenomes, Fig.