https://divingintogeneticsandgenomics.com/tags/diffbind/ Recent content in Diffbind on Chatomics Hugo -- gohugo.io en-us © 2018 Ming 'Tommy' Tang Wed, 20 Apr 2016 10:42:00 -0700 https://divingintogeneticsandgenomics.com/archive/library-size-normalization-for-chip-seq/ Wed, 20 Apr 2016 10:42:00 -0700 https://divingintogeneticsandgenomics.com/archive/library-size-normalization-for-chip-seq/ I have discussed how to use DESeq2 to do differential binding for ChIP-seq at here. I am experimenting DiffBind to do the same thing, which internally uses EdgR, DESeq and DESeq2. The author Rory Stark is very responsive on the bioconductor support site and has answered several of my questions. Today, I am going to keep a note here for normalizing the ChIP-seq data. If one compares ChIP-seq versus RNA-seq data, they are in the end all counts data.